Welcome at the Competence Centre for Genomic Analysis (CCGA) Kiel

The CCGA is the sequencing Core Facility of the Christian-Albrechts-University Kiel (CAU) and the University Hospital Schleswig-Holstein (UKSH), located at the Institute of Clinical Molecular Biology.

We were one of four DFG-funded Next Generation Sequencing (NGS) centres with the mission to strengthen the biological/medical infrastructure in Germany. Continuous infrastructure funding is now secured via the state of Schleswig-Holstein.

Our services comprise short and long read sequencing technologies for different applications as well as bioinformatics support via our partners @BIP.

Instrumentation

Illumina, long read	#	Output range/all FlowCells [Gb]	Output range/all FlowCells [million read pairs]	Lanes/FlowCell	Max read length
NovaSeqX-Plus	2	164 - 7.600	1.500 - 25.000	2 - 8	2 x 300 bp
NovaSeq6000 (to be phased out)	2	65 - 2.400	750 - 8.000	2 - 4	2 x 250 bp
NextSeq1000	1	10 - 240	100 - 400	1	2 x 300 bp
MiSeq i100	1	1.5 - 25	5 - 25	1	2 x 500 bp
MiSeq	2	0.3 - 13.2	1 - 22	1	2 x 300 bp
PacBio	#	Output SMRTcell [HiFi Gb]	Output range SMRTcell [HiFi million reads]	SMRTCells per run	Max read length
Revio	1	32 - 120	4 - 8	4 - 8	25 kb
Sequel IIe (to be phased out)	1	5 - 30	1 - 3	4 - 8	25 kb
10x Genomics	#	Output
CytAssist	1	Illumina
Xenium	1	Imaging
Chromium X	1	Illumina + PacBio

Library types

Applications	Library types	Contact	Where to order
Transcriptomics / Metatranscriptomics	Illumina stranded mRNA, Illumina stranded total RNA incl. RiboZero depletion, NextFlex v4 smallRNA, Kinnex full length RNA, low input and FFPE on request	ccga@ikmb.uni-kiel.de	CCGA
Genomics / Metagenomics	Illumina DNA prep, Illumina DNA prep PCRfree, NEBNext Ultra II FS, SMRTBell HiFi 3.0, Ampli-Fi HiFi 3.0	ccga@ikmb.uni-kiel.de	CCGA
Epigenetics	SMRTBell HiFi 3.0, NEBNext EMseq (whole genome or reduced representation), Illumina 5-base	ccga@ikmb.uni-kiel.de	CCGA
SingleCell Transcriptomics & Epigenetics	10x portfolio, custom preparations incl. methylation - before starting a project, get in touch	ccga@ikmb.uni-kiel.de j.pimentabernardes@ikmb.uni-kiel.de	CCGA
Spatial Transcriptomics	10x portfolio - before starting a project, get in touch	ccga@ikmb.uni-kiel.de j.pimentabernardes@ikmb.uni-kiel.de	CCGA
Microbiome analysis	in house amplicon protocols: archeal 16S v3v4, bacterial 16S v1v2 or v3v4, fungal ITS2	c.bang@ikmb.uni-kiel.de	Intake Microbiome Analysis
Immunogenetics	Cellecta AIR RNA or DNA TCR and/or BCR profiling, in house HLA profiling	ccga@ikmb.uni-kiel.de b.loescher@ikmb.uni-kiel.de	CCGA
Proteomics	Olink HT, Olink Reveal	genotype_ta@ikmb.uni-kiel.de	Intake Genotyping

Details regarding different FlowCell types and library preparation methods as well as automation and quality control can be found here.