The Blue Pippin is an automated gel extraction system with a pulse field function. It can be used to extract a certain size range or to remove fragments smaller than a defined cut off. The size range for fragment recovery ranges from 100 bp to 50 kb.
The 10x Chromium controller is a microfluidics device optimized for single cell transcriptomics, immuno-profiling and cell surface protein characterization (CITEseq). Individual labeling of single cells is achieved by "Gel bead in Emulsion" (GEM) - technology. This means, each cell is encapsulated in a droplet together with a GEM (gel bead). That GEM contains everything necessary for the following cDNA synthesis including an individual barcode, that is attached to every cDNA fragment originating from that gel bead. Thus, it is possible to prepare a “pooled” library from all individually labelled fragments and still be able to track their origin back to one cell.
The Sciclone G3 is a highly flexible, (ultra) high throughput liquid handling workstation that is configured for NGS solutions in our lab.
The NGS express liquid handling workstation is optimized for medium throughput library preparation and normalization.
The Zephyr G3 NGS is a high throughput liquid handling workstation that can be used for library prep, normalization and pooling of samples.
The Covaris instruments allow for (unbiased) DNA shearing with ultrasonication in variable throughputs.
The Diagenode Megaruptor allows for precise shearing of DNA for long read sequencing projects.
The Femto pulse is a (pulse field) capillary electrophoresis device which allows quality and quantity assessment in high throughput for DNA and RNA. We have one Femto pulse for entry QC and library QC, especially for ultra low input samples (0,05 ng/µl detectable) and high molecular weight applications. The Femto pulse can assess genomic DNA up to a size range > 165 kb with great resolution and helps to assess the ideal sequencing parameters for PacBio libraries.
The TapeStation is a capillary electrophoresis device which allows quality and quantity assessment in high throughput for DNA and RNA. We have two TapeStation 4200 for entry QC and library QC. The TapeStation can assess genomic DNA up to a size range > 60 kb but is not able to resolve the sizes at a high resolution.
The LabChip GX Touch HT is also a capillary electrophoresis device with a higher sensitivity and higher throughput than the TapeStation. The LabChip is available for DNA and RNA and is especially suited for FFPE samples or cell free DNA.
The VICTOR Nivo is a plate reader with a broad spectrum of filters to allow for many different detection methods. We use it mainly for high throughput fluorescence-based quantification of dsDNA and RNA.
The Qubit is an easy to use, low throughput fluorescence-based quantification device, which can be used for DNA and RNA quantification.
We use the Nanodrop to assess the purity of DNA and RNA samples, especially for PacBio sequencing. Unless the DNA/RNA is ultra-clean, the Nanodrop is not suited for accurate quantification.